human cthrc1 protein  (MedChemExpress)

Journal: Experimental and Therapeutic Medicine

Article Title: Collagen triple helix repeat containing 1 promotes endometrial cancer cell migration by activating the focal adhesion kinase signaling pathway

doi: 10.3892/etm.2020.8833

Figure Lengend Snippet: CTHRC1 is commonly upregulated in EC. (A) Immunohistochemical analysis of CTHRC1 expression in adjacent normal endometrium (n=11) and EC (n=18) tissues. Magnification, x200. No or weak expression of CTHRC1 was observed in normal endometrium tissues. Strong cell membrane and cytoplasmic expression of CTHRC1 was observed in most EC tissues. (B) Relative RNA expression levels of CTHRC1 in EC tissues (n=14) and adjacent normal tissues (n=14) were assessed by reverse transcription-quantitative PCR analysis. (C) ELISA analysis of levels of secreted CTHRC1 in the plasma of EC patients or normal females (EC group, n=39; normal group, n=12). (D) Relative mRNA expression of CTHRC1 in the normal and EC groups in TCGA Uterine Corpus Endometrial Cancer database (EC tissues, n=546; normal tissues, n=35). (E) Kaplan-Meier curves of survival for EC patients with high or low CTHRC1 expression (high CTHRC1 expression, n=162; low CTHRC1 expression, n=216). Experiments were repeated in triplicate. Data are presented as the mean ± SD. * P<0.05, *** P<0.001. CTHRC1, collagen triple helix repeat containing 1; EC, endometrial cancer; TCGA, The Cancer Genome Atlas.

Article Snippet: Ishikawa and ECC1 cells cultured in complete medium treated with PBS or recombinant protein CTHRC1 (100 μg/ml; Abcam), CTHRC1 with the FAK signaling inhibitor defactinib (1 μM; cat. no. HY-12289; MedChemExpress) or recombinant protein CTHRC1 (100 μg/ml) with the FAK signaling inhibitor Y15 (1 μM; cat. no. HY-12444; MedChemExpress) were termed Control, +CTHRC1, +CTHRC1 +Defactinib or +CTHRC1 +Y15.

Techniques: Immunohistochemical staining, Expressing, Membrane, RNA Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Experimental and Therapeutic Medicine

Article Title: Collagen triple helix repeat containing 1 promotes endometrial cancer cell migration by activating the focal adhesion kinase signaling pathway

doi: 10.3892/etm.2020.8833

Figure Lengend Snippet: Overexpression of CTHRC1 promotes the migration of EC cells in vitro . (A) Western blot analysis of the expression levels of CTHRC1 in Ishikawa and ECC1 cells. (B) Proliferation of Ishikawa and ECC1 cells with CTHRC1-OE was confirmed by CCK-8 assays. (C) Migration of Ishikawa and ECC1 cells with CTHRC1-OE confirmed by Transwell assays. Magnification, x200. (D) Migration of Ishikawa and ECC1 cells with CTHRC1-OE confirmed by wound healing assays. Magnification, x50. (E) Invasion of Ishikawa and ECC1 cells with CTHRC1-OE confirmed by Transwell assays with Matrigel. Magnification, x200). (F) Focal adhesion and actin remodeling were analyzed by immunofluorescence. Magnification, x100. Vinculin was marked by red fluorescence and phalloidin, an actin stain, was marked by green fluorescence, while the nucleus was stained with DAPI (blue). The results were determined in triplicate. Data are presented as the mean ± SD. * P<0.05, ** P<0.01. CTHRC1, Collagen triple helix repeat containing 1; EC, endometrial cancer; CCK-8, Cell Counting Kit-8; OE, overexpression.

Article Snippet: Ishikawa and ECC1 cells cultured in complete medium treated with PBS or recombinant protein CTHRC1 (100 μg/ml; Abcam), CTHRC1 with the FAK signaling inhibitor defactinib (1 μM; cat. no. HY-12289; MedChemExpress) or recombinant protein CTHRC1 (100 μg/ml) with the FAK signaling inhibitor Y15 (1 μM; cat. no. HY-12444; MedChemExpress) were termed Control, +CTHRC1, +CTHRC1 +Defactinib or +CTHRC1 +Y15.

Techniques: Over Expression, Migration, In Vitro, Western Blot, Expressing, CCK-8 Assay, Immunofluorescence, Fluorescence, Staining, Cell Counting

Journal: Experimental and Therapeutic Medicine

Article Title: Collagen triple helix repeat containing 1 promotes endometrial cancer cell migration by activating the focal adhesion kinase signaling pathway

doi: 10.3892/etm.2020.8833

Figure Lengend Snippet: Recombinant CTHRC1-mediated promotion of EC cell migration in vitro . (A) Proliferation of +CTHRC1 Ishikawa and ECC1 cells confirmed by CCK-8 assays. (B) Migration of Ishikawa and ECC1 cells with +CTHRC1 confirmed by Transwell assays. Magnification, x200. (C) Migration of Ishikawa and ECC1 cells with +CTHRC1 confirmed by wound healing assays. Magnification, x50. (D) Invasion of Ishikawa and ECC1 cells with +CTHRC1 confirmed by Transwell assays with Matrigel. Magnification, x200. (E) Focal adhesion and cyto-actin remodeling were analyzed by immunofluorescence. Magnification, x100. Vinculin was marked by red fluorescence and phalloidin was marked by green fluorescence, while the nucleus was stained with DAPI (blue). The experiments were performed in triplicate, data are presented as the mean ± SD. * P<0.05. CTHRC1, Collagen triple helix repeat containing 1; EC, endometrial cancer; CCK-8, cell counting kit-8; +CTHRC1, cells with recombinant CTHRC1 protein treatment.

Article Snippet: Ishikawa and ECC1 cells cultured in complete medium treated with PBS or recombinant protein CTHRC1 (100 μg/ml; Abcam), CTHRC1 with the FAK signaling inhibitor defactinib (1 μM; cat. no. HY-12289; MedChemExpress) or recombinant protein CTHRC1 (100 μg/ml) with the FAK signaling inhibitor Y15 (1 μM; cat. no. HY-12444; MedChemExpress) were termed Control, +CTHRC1, +CTHRC1 +Defactinib or +CTHRC1 +Y15.

Techniques: Recombinant, Migration, In Vitro, CCK-8 Assay, Immunofluorescence, Fluorescence, Staining, Cell Counting

Journal: Experimental and Therapeutic Medicine

Article Title: Collagen triple helix repeat containing 1 promotes endometrial cancer cell migration by activating the focal adhesion kinase signaling pathway

doi: 10.3892/etm.2020.8833

Figure Lengend Snippet: CTHRC1 mediates the migration of EC cells via the FAK signaling pathway. (A) Western blot analysis of P-FAK Tyr397 and T-FAK in cells with CTHRC1-OE. (B) Western blot analysis of P-FAK Tyr397 and T-FAK in the +CTHRC1 group or +CTHRC1 cells with defactinib or Y15. Migration confirmed by (C) Transwell assays, magnification, x200; and (D) wound healing assays. Magnification, x50. (E) The high expression of CTHRC1 in EC cells and plasma promoted EC cell migration by activating the FAK signaling pathway. The FAK signaling inhibitors, defactinib and Y15, could reverse the enhanced migration mediated by CTHRC1. The experiments were performed in triplicate. Data are presented as the mean ± SD. * P<0.05, ** P<0.01, *** P<0.001. CTHRC1, collagen triple helix repeat containing 1; EC, endometrial cancer; FAK, focal adhesion kinase; P-FAK, phospho-FAK; T-FAK, Total-FAK; OE, overexpression.

Article Snippet: Ishikawa and ECC1 cells cultured in complete medium treated with PBS or recombinant protein CTHRC1 (100 μg/ml; Abcam), CTHRC1 with the FAK signaling inhibitor defactinib (1 μM; cat. no. HY-12289; MedChemExpress) or recombinant protein CTHRC1 (100 μg/ml) with the FAK signaling inhibitor Y15 (1 μM; cat. no. HY-12444; MedChemExpress) were termed Control, +CTHRC1, +CTHRC1 +Defactinib or +CTHRC1 +Y15.

Techniques: Migration, Western Blot, Expressing, Clinical Proteomics, Over Expression

Journal: Remote Sensing

Article Title: Typhoon/Hurricane–Generated Wind Waves Inferred from SAR Imagery

doi: 10.3390/rs10101605

Figure Lengend Snippet: Figure 1. CTHRC1 expression is increased in human OA compared with normal tissues. (A) IL‑1β and (B) CTHRC1 levels in the joint fluid of patients with OA and control patients were assessed using ELISA. **P<0.01 vs. normal. CTHRC1, collagen triple helix repeat containing 1; OA, osteoarthritis; IL, interleukin.

Article Snippet: IL-1β and cTHRc1 expression in the joint fluid of patients with OA was determined using cTHRc1 (cat. no. cSB-EL006162HU; cusabio Biotech co., Ltd., college Park, Md, USA) and IL-1β (cat. no. 583311; cayman chemical company, Ann Arbor, MI, USA) ELISA kits according to the manufacturer's protocol.

Techniques: Expressing, Control, Enzyme-linked Immunosorbent Assay

Journal: Remote Sensing

Article Title: Typhoon/Hurricane–Generated Wind Waves Inferred from SAR Imagery

doi: 10.3390/rs10101605

Figure Lengend Snippet: Figure 3. CTHRC1 upregulation increases JNK1/2 activation. IL‑1β‑induced chondrocytes were transfected with pLVX‑Puro‑CTHRC1 for 24 h and CTHRC1 expression was assessed using (A) reverse transcription‑quantitative polymerase chain reaction and (B and C) western blotting. The expression of p‑JNK1/1 and JNK1/2 in IL‑1β‑induced chondrocytes following pLVX‑Puro‑CTHRC1 transfection or SP600125 treatment was assessed using (D) western blotting and (E) quantified. **P<0.01 vs. vector and ##P<0.01 vs. pLVX‑Puro‑CTHRC1. CTHRC1, collagen triple helix repeat containing 1; JNK, c‑Jun N‑terminal kinase; IL, interleukin; p, phosphorylated.

Article Snippet: IL-1β and cTHRc1 expression in the joint fluid of patients with OA was determined using cTHRc1 (cat. no. cSB-EL006162HU; cusabio Biotech co., Ltd., college Park, Md, USA) and IL-1β (cat. no. 583311; cayman chemical company, Ann Arbor, MI, USA) ELISA kits according to the manufacturer's protocol.

Techniques: Activation Assay, Transfection, Expressing, Polymerase Chain Reaction, Western Blot, Plasmid Preparation

Journal: Remote Sensing

Article Title: Typhoon/Hurricane–Generated Wind Waves Inferred from SAR Imagery

doi: 10.3390/rs10101605

Figure Lengend Snippet: Figure 2. IL‑1β increases CTHRC1 expression and inhibits chondrocyte proliferation. (A) Chondrocytes were collected from rat articular cartilage and the expression of Collagen II and SOX9 was measured using IHC. (B) Rat chondrocytes were treated with 5, 10 and 20 ng/ml IL‑1β and the expression of CTHRC1 was assessed using western blotting. (C) Cell proliferation was measured using a CCK‑8 assay. **P<0.01 vs. control. IL, interleukin; CTHRC1, collagen triple helix repeat containing 1; SOX9, Sry‑type high mobility group‑box; IHC, immunohistochemistry; CCK‑8, Cell Counting Kit‑8.

Article Snippet: IL-1β and cTHRc1 expression in the joint fluid of patients with OA was determined using cTHRc1 (cat. no. cSB-EL006162HU; cusabio Biotech co., Ltd., college Park, Md, USA) and IL-1β (cat. no. 583311; cayman chemical company, Ann Arbor, MI, USA) ELISA kits according to the manufacturer's protocol.

Techniques: Expressing, Western Blot, CCK-8 Assay, Control, Immunohistochemistry

Journal: Remote Sensing

Article Title: Typhoon/Hurricane–Generated Wind Waves Inferred from SAR Imagery

doi: 10.3390/rs10101605

Figure Lengend Snippet: Figure 4. CTHRC1 upregulation induces chondrocyte apoptosis. (A and B) Chondrocyte apoptosis was assessed by flow cytometry analysis. (C) Changes in caspase‑3 activity were investigated using spectrophotometry. (D and E) The expression of Bcl‑2, Bax, cleaved caspase‑3, PARP‑1 and MMP‑13 was studied using western blotting. **P<0.01 vs. vector and ##P<0.01 vs. pLVX‑Puro‑CTHRC1. CTHRC1, collagen triple helix repeat containing 1; Bcl‑2, B‑cell lymphoma‑2; Bax, Bcl‑2‑associated X protein; PARP, poly ADP ribose polymerase; MMP, matrix metalloproteinase.

Article Snippet: IL-1β and cTHRc1 expression in the joint fluid of patients with OA was determined using cTHRc1 (cat. no. cSB-EL006162HU; cusabio Biotech co., Ltd., college Park, Md, USA) and IL-1β (cat. no. 583311; cayman chemical company, Ann Arbor, MI, USA) ELISA kits according to the manufacturer's protocol.

Techniques: Flow Cytometry, Activity Assay, Spectrophotometry, Expressing, Western Blot, Plasmid Preparation

Journal: Remote Sensing

Article Title: Typhoon/Hurricane–Generated Wind Waves Inferred from SAR Imagery

doi: 10.3390/rs10101605

Figure Lengend Snippet: Figure 5. CTHRC1 downregulation inhibits JNK1/2 activation. IL‑1β‑induced chondrocytes pLKO.1‑CTHRC1‑shRNA were transfected for 24 h and CTHRC1 expression was assessed using (A) reverse transcription‑quantitative polymerase chain reaction and (B and C) western blotting. (D and E) The expression of p‑JNK1/2 and JNK1/2 in IL‑1β‑induced chondrocytes following shRNA‑CTHRC1 transfection or SP600125 treatment was assessed using western blotting. **P<0.01 vs. control and ##P<0.01 vs. IL‑1β. CTHRC1, collagen triple helix repeat containing 1; JNK, c‑Jun N‑terminal kinase; IL, interleukin; sh, short hairpin; NC, negative control; p, phosphorylated.

Article Snippet: IL-1β and cTHRc1 expression in the joint fluid of patients with OA was determined using cTHRc1 (cat. no. cSB-EL006162HU; cusabio Biotech co., Ltd., college Park, Md, USA) and IL-1β (cat. no. 583311; cayman chemical company, Ann Arbor, MI, USA) ELISA kits according to the manufacturer's protocol.

Techniques: Activation Assay, Transfection, Expressing, Polymerase Chain Reaction, Western Blot, Control, Negative Control

Journal: Remote Sensing

Article Title: Typhoon/Hurricane–Generated Wind Waves Inferred from SAR Imagery

doi: 10.3390/rs10101605

Figure Lengend Snippet: Figure 6. CTHRC1 downregulation inhibits chondrocyte apoptosis. (A) Chondrocyte apoptosis was assessed by flow cytometry and (B) statistically analyzed. (C, D and E) The expression of Bcl‑2, Bax, cleaved caspase‑3, PARP‑1 and MMP‑13 was assessed using reverse transcription‑quantitative polymerase chain reaction and western blotting. (F) Changes in caspase‑3 activity were measured by spectrophotometry. **P<0.01 vs. control and ##P<0.01 vs. IL‑1β. CTHRC1, collagen triple helix repeat containing 1; Bcl‑2, B‑cell lymphoma‑2; Bax, Bcl‑2‑associated X protein; PARP, poly ADP ribose polymerase; MMP, matrix metalloproteinase.

Article Snippet: IL-1β and cTHRc1 expression in the joint fluid of patients with OA was determined using cTHRc1 (cat. no. cSB-EL006162HU; cusabio Biotech co., Ltd., college Park, Md, USA) and IL-1β (cat. no. 583311; cayman chemical company, Ann Arbor, MI, USA) ELISA kits according to the manufacturer's protocol.

Techniques: Flow Cytometry, Expressing, Polymerase Chain Reaction, Western Blot, Activity Assay, Spectrophotometry, Control